Overall Categorization
Attributes of FASTQ
Alphabet Frequency & GC content
eel_PE400
Attributes of FASTQ
Name Value InputFile eel_PE400.fastq #Read 1,000,000 #Base 192,332,072 AvgReadLen 192.33 MinReadLen 35 MaxReadLen 201
Alphabet Frequency & GC content
Name Count Freq% A 54,810,615 28.50% C 41,525,489 21.59% G 40,954,335 21.29% T 55,038,650 28.62% N 2,983 0.00% GC% - 42.88%
Distribution of Bases' Quality Values
Name AreaFreq Q30 & above 92.6% Q20-Q29 5.6% Q15-Q19 1.0% < Q15 0.8%
Distribution of Reads' MinimalQ Values
Name AreaFreq % of reads whose bases are all Q20 & above 60.7% % of reads whose bases are all Q15 & above 73.0% % of reads whose bases are all Q10 & above 77.6%
Coverage of Reads with Sufficient High-Quality Bases
Name Coverage of reads Remark % of High-quality reads
Coverage of reads that 100% of their bases with Q20 & above 60.7% %HighQ(20) >= 100%
(i.e., MinimaQ>=20) Coverage of reads that >= 95% of their bases with Q20 & above 87.6% %HighQ(20) >= 95% Coverage of reads that >= 90% of their bases with Q20 & above 92.9% %HighQ(20) >= 90% Coverage of reads that >= 90% of their bases with Q15 & above 97.6% %HighQ(15) >= 90% % of Poor-quality reads
Coverage of reads that > 10% of their bases with Q14 & less 2.4% 1 - {%HighQ(15) >= 90%}
Attributes of FASTQ
| Name | Value |
|---|---|
| InputFile | eel_PE400.fastq |
| #Read | 1,000,000 |
| #Base | 192,332,072 |
| AvgReadLen | 192.33 |
| MinReadLen | 35 |
| MaxReadLen | 201 |
Alphabet Frequency & GC content
| Name | Count | Freq% |
|---|---|---|
| A | 54,810,615 | 28.50% |
| C | 41,525,489 | 21.59% |
| G | 40,954,335 | 21.29% |
| T | 55,038,650 | 28.62% |
| N | 2,983 | 0.00% |
| GC% | - | 42.88% |
Distribution of Bases' Quality Values
| Name | AreaFreq |
|---|---|
| Q30 & above | 92.6% |
| Q20-Q29 | 5.6% |
| Q15-Q19 | 1.0% |
| < Q15 | 0.8% |
Distribution of Reads' MinimalQ Values
| Name | AreaFreq |
|---|---|
| % of reads whose bases are all Q20 & above | 60.7% |
| % of reads whose bases are all Q15 & above | 73.0% |
| % of reads whose bases are all Q10 & above | 77.6% |
Coverage of Reads with Sufficient High-Quality Bases
| Name | Coverage of reads | Remark |
|---|---|---|
| % of High-quality reads Coverage of reads that 100% of their bases with Q20 & above | 60.7% | %HighQ(20) >= 100% (i.e., MinimaQ>=20) |
| Coverage of reads that >= 95% of their bases with Q20 & above | 87.6% | %HighQ(20) >= 95% |
| Coverage of reads that >= 90% of their bases with Q20 & above | 92.9% | %HighQ(20) >= 90% |
| Coverage of reads that >= 90% of their bases with Q15 & above | 97.6% | %HighQ(15) >= 90% |
| % of Poor-quality reads Coverage of reads that > 10% of their bases with Q14 & less | 2.4% | 1 - {%HighQ(15) >= 90%} |
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